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Figure 5 | Journal of Hematology & Oncology

Figure 5

From: miR-363-5p regulates endothelial cell properties and their communication with hematopoietic precursor cells

Figure 5

miR-363-5p interaction with TIMP-1 UTR. (A) Schematic view of the TIMP1 construct into pMIR-REPORT showing the two predicted binding sites in TIMP1 3’UTR. Sequence alignment of miR-363-5p and the 3’UTR TIMP1 is shown. Nucleotide mutations achieved by site-directed mutagenesis are colored in red. (B) Renilla luciferase activity normalized to Firefly activity of HUVEC 48 h post-transfection with pre-miR-363-5p relative to scramble control. Relative luciferase activity of wild-type (WT) 3’UTR constructs and mutation in the predicted site 1 (MUT1), site 2 (MUT2) and both sites mutated (MUT1 + 2) are shown. Errors bars are s.e.m. from three independent transfection experiments. * P ≤ 0.05, *** P ≤ 0.001 by Student’s t test.

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