Figure 5

CK2α and CK2β silencing potentiates daunorubicin-induced AML cell apoptosis. (A) Graph summarizing the results of real time quantitative PCR analysis of CK2α and CK2β mRNA in ML-2 cells transfected with no target scrambled oligos, CK2α or CK2β-directed siRNAs. (B) Histogram graph (top) and dot plot graphs (bottom) summarizing annexin V and FACS analysis of the rate of apoptosis of ML-2 cells upon transfection with no target scrambled, CK2α or CK2β-directed siRNAs without or with exposure to daunorubicin 0.15 μM or 0.25 μM. (C) Representative immunoblot analysis of PARP cleavage and CK2α and CK2β protein levels of ML-2 cells transfected with no target scrambled, CK2α or CK2β-directed siRNAs without or with exposure to daunorubicin 0.15 μM or 0.25 μM. In the indicated experiments data represent mean ± SD, n = 4. * indicates p < 0.05.