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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Immunoglobulin E induces VEGF production in mast cells and potentiates their pro-tumorigenic actions through a Fyn kinase-dependent mechanism

Figure 6

IgE improves the pro-tumorigenic properties of MC. ( A and B ) Participation of MC on IgE-induced melanoma tumor growth. C57BL6/J (WT), Wsh and Wsh Rec WT mice were treated with a single i.v. administration of saline (−IgE) or monoclonal anti-DNP IgE (750 ng/mouse; +IgE). Twenty four hours later, mice were s.c. inoculated with B16 melanoma cells (+B16) in one ear pinna and tumor weight was determined four weeks after inoculation. Representative pictures of tumors from WT, Wsh and Wsh Rec WT mice after four weeks of inoculation are shown in A. Mean tumor weight of WT, Wsh and Wsh Rec WT mice. Mean ± SEM (n = 7-12). *, P < 0.05 versus IgE-treated mice; #, P < 0.05 versus WT IgE-treated mice; &, P < 0.05 versus Wsh IgE-treated mice. ( C ) Histological analysis of tissue biopsies. Ear pinna sections (2.5 μm) were stained with H&E and TB. Blood vessels are indicated by arrows and MC by arrowheads. Pictures are representative images (n = 2-3). Scale bar = 20 μm. ( D ) Quantification of blood vessels in ear pinna from treated animals. Mean ± SEM (n = 3). *, P < 0.05 versus WT-IgE + B16; α, P < 0.001 versus WT-IgE + B16; β, P < 0.001 versus WT + IgE + B16; λ, P < 0.05 versus WT-IgE + B16; δ, P < 0.001 versus Wsh-IgE + B16; ø, P < 0.001 versus Wsh Rec WT-IgE + B16; Ω, P < 0.001 versus Wsh + IgE + B16; Ψ, P < 0.01 versus WT + IgE + B16; ∑, P < 0.05 versus Wsh-IgE + B16.

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