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Figure 2 | Journal of Hematology & Oncology

Figure 2

From: Immunoglobulin E induces VEGF production in mast cells and potentiates their pro-tumorigenic actions through a Fyn kinase-dependent mechanism

Figure 2

IgE-induced VEGF protein but not mRNA accumulation is affected in Fyn −/− BMMCs. ( A and B ) Intracellular VEGF accumulation after IgE treatment of MC. Two million WT and Fyn −/− BMMCs were stimulated with 1000 ng of IgE for eight h at 37°C. Cells were collected in a cytospin and slides were incubated in the presence of an anti-VEGF antibody (red) and DAPI (blue). Samples were processed to detect intracellular VEGF by confocal microscopy. A representative picture obtained from at least three independent experiments is shown. Scale bar = 10 μm. Mean fluorescence intensity values of VEGF signal obtained from experiments shown in A (n = 3) are represented in B. *, P < 0.0001 compared with IgE 0 ng; &, P < 0.001 compared with WT. Two way ANOVA post hoc Student-Newman-Keuls. ( C and D ) VEGF mRNA expression in IgE-stimulated MC. Total mRNA was purified from WT or Fyn −/− BMMCs treated with 1000 ng IgE and VEGF164 mRNA was amplified by RT-PCR. GAPDH amplification is shown as a control. Densitometric quantification of VEGF164 mRNA (n = 2) is shown in D.

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