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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: L1cam promotes tumor progression and metastasis and is an independent unfavorable prognostic factor in gastric cancer

Figure 6

PI3K/Akt signaling is involved in L1cam stimulated gastric cancer cell migration and invasion. (A) Western blot analysis of whole- cell lysates using anti-phospho-Akt (Ser473) or anti-Akt antibody. (B) Western blot analysis in from HGC27 cells expressing L1cam or empty vector after pretreatment with 20 μM LY294002 for 30 min, using anti-phospho-Akt (Ser473) or anti-Akt antibody. (C) Migratory and invasive abilities of HGC27 cells expressing L1cam or the empty vector evaluated by Transwell assay after pretreatment with LY294002. (D) Western blot analysis of whole-cell lysates from HGC27 cells expressing L1cam or the empty vector 24 h after transfection with AKT siRNA or a scrambled siRNA, using anti-phospho-Akt (Ser473) or anti-Akt antibody. (E) Migratory and invasive abilities of HGC27 cells expressing L1cam or the empty vector as evaluated by Transwell assay after transfection with Akt siRNA or the scrambled siRNA. (F) SGC7901 cells were injected into the flank of nude mice, LY294002 (25 mg/kg) were intraperitoneally injected into the nude mice every four days, tumor volumes were measured at indicated days. (G) Tumors formed by different cells (SGC7901/Scramble or SGC7901/sh-L1cam, SGC7901/DMSO or SGC7901/LY294002) were collected and protein was extracted. Western blot analysis was conducted to detect the Akt and phospho-Akt level. Photomicrographs are at 100×. Bars correspond to mean ± SD of three independent experiments.

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