Figure 7
From: Activated N-Ras signaling regulates arterial-venous specification in zebrafish
Loss of arterial specification by hNRASD12 expression before arterial-venous segregation and onset of circulation. (A-H) WISH analysis of arterial (ephrinB2+, hRT+) and venous (flt4+, dab2+) cells in the trunk of control (A, C, E, G) and hNRASD12 embryos (B, D, F, H) at 20 hpf respectively. (I-L) 2-color WISH analysis with digoxigenin-labeled ephrinB2 (white arrows) and fluorescein-labeled flt4 (white triangles) in control (I, J) and hNRASD12 embryos (K, L).