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Figure 6 | Journal of Hematology & Oncology

Figure 6

From: Activated N-Ras signaling regulates arterial-venous specification in zebrafish

Figure 6

Expression of hNRASD12 promotes venous specification at the expense of arterial fate during embryonic endothelial differentiation. (A) Quantitation of total endothelial cells by flow cytometry analysis on the control;flk1:GFP and hNRASD12;flk1:GFP embryos at 28 hpf. Representative results obtained from three independent experiments. 2 tailed t-test of frequency of endothelial cells in hNRASD12;flk1:GFP embryos vs control;flk1:GFP embryos shows no significant difference. (B-E’) WISH analysis of ephrinB2+ and hRT+ arterial cells in the trunk of control (B-E, blue triangles) and hNRASD12 embryos (B’-E’, red triangles) at 28 hpf. (F-I’) WISH analysis of flt4+ and ephB4+ venous cells in the trunk of control (F-I) and hNRASD12 embryos (F’-I’) at 28 hpf. The yellow square brackets mark the width of the venous strip in the trunk. (J-K’) 2-color WISH analysis with digoxigenin-labeled ephrinB2 (white arrows) and fluorescein-labeled flt4 (white triangles) in control (J, K) and hNRASD12 embryos (J’, K’) respectively at 28 hpf.

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