Figure 2

HIF-1α and Notch1 expression are down-regulated in T-ALL cells transfected with specific siRNA. Jurkat and Sup-T1 T-ALL cells were transfected with HIF-1α siRNA, Notch1 siRNA or control siRNA and cultured under normoxic conditions. (A) qRT-PCR shows mRNA levels of HIF-1α and Notch1, normalized to β-Actin and presented as relative mRNA expression level. *, P < 0.01. Results are an average of three independent experiments. (B) Western blot analysis of HIF-1α and Notch1 ICN expression in Jurkat and Sup-T1 cells transfected with HIF-1α or Notch1 siRNA. Results shown are representative of at least three independent experiments.