Table 2 Sense and antisense primers for amplification of the tested genes with real-time PCR
mRNA target | Sense (5′) primers | Antisense (3′) primers |
|---|---|---|
xIAP | 5′-CCATATACCCGAGGAACCCT-3′ | 5′-TTTCCACCACAACAAAAGCA-3′ |
Mcl-1 | 5′-AAAAGCAAGTGGCAAGAGGA-3′ | 5′-TTAATGAATTCGGCGGGTAA-3′ |
Bcl-2 | 5′-AAGATTGATGGGATCGTTGC-3′ | 5′-TGTGCTTTGCATTCTTGGAC-3′ |
cIAP-1 | 5′-GCTCAGTAACTGGGAACCAAA-3′ | 5′-ATCATTGCGACCCACATAATA-3′ |
Survivin | 5′- CAGATTTGAATCGCGGGACCC-3′ | 5′- CCAAGTCTGGCTCGTTCTCAG-3′ |
IGF-1R | 5′-TTAAAATGGCCAGAACCTGAG-3′ | 5′-ATTATAACCAAGCCTCCCAC-3′ |
PIK3CD | 5′-CGGGACACAGGGAAGTTCAGGT-3′ | 5′-TAAGGAGTCAGGCCAGGGCGG-3′ |
18 s rRNA | 5′-GTAACCCGTTGAACCCCATT-3′ | 5′-CCATCCAATCGGTAGTAGCG-3′ |