Figure 1
SNS-032 potently inhibits cell growth in AML cell lines and primary AML blasts. (A) IC50 of cell lines tested in 24 h by an MTT assay. Data show mean ± s.d. from three independent experiments, each performed in triplicates. The comments of cell lines are shown. (B) After treatment with 50, 100, or even 200 nM SNS-032 for 7 days, the ability of colony formation of all tested cell lines were evaluated in methylcellulose cultures. All data represent an independent experiment from three repeated tests with similar results. Each point is the mean of triplicates; bar, ± s.d. * represent P < 0.0001; #P = 0.31. (C) IC50 of primary AML cells from the patients with different French-American-British (FAB) classification subtypes was examined in 48 h by colorimetric assay. (D) Primary leukemic cells from 4 patients with newly diagnostic AML were treated with SNS-032 (200 nM) and then cultured in methylcellulose medium containing rhSCF, rhGM-CSF and rhIL-3 for 7 days. Colonies were counted under a microscope. * P = 0.0009; ** P = 0.0005; # P < 0.0001. (E) Human normal mononuclear cells obtained from bone marrow of 5 healthy volunteers were incubated with or without SNS-032, and then cultured in methylcellulose medium for 12 days to detect colony formation. * represent P > 0.05; # P = 0.0052.