Figure 4

Expression of SRSF1 and PRMT1 in cell lines before and after treatment with chemotherapeutics. Nalm-6 cells (A), Reh cells (B) and Normal B (C) cells were treated with VCR, Ara-c and NS (negative control) for 24 and 48 hours, respectively. Cell lysates were probed with anti-SRSF1 and anti-PRMT1 antibodies, and GAPDH was used as a loading control. Nalm-6 is a pre-B ALL cell line with no fusion gene, while Reh is a pre-B cell line with the TEL-AML1 fusion gene. The normal B (NB) cell line is derived from EBV-transformed human B cells.