Figure 3

Kinase assays with GST-FLT3S identify potent FLT3 inhibitors. A. Tyrosine kinase activity of wild type and mutation forms of FLT3 were analyzed with 0.2 mg/ml GST-FLT3S in the presence of indicated tyrosine kinase inhibitors at 0.3 μM. Phosphorylation of GST-FLT3S was detected by using anti-phosphotyrosine antibody. B. Quantitative representation of GST-FLT3S phosphorylation activity. Error bars denote standard deviation (n ≥ 3). The stock solutions of inhibitors were made in dimethyl sulfoxide (DMSO), which was used as control. Note the strong inhibitory effects of sunitinib, sorafenib, and lestaurtinib.