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Figure 3 | Journal of Hematology & Oncology

Figure 3

From: Deprivation of arginine by recombinant human arginase in prostate cancer cells

Figure 3

Determination of apoptosis by TUNEL assay. Cells were incubated with increasing concentrations of rhArg for 36 h before TUNEL assay and subsequent analysis by flow cytometer. Purple, green, pink, blue, and orange histograms indicate levels of DNA fragmentation with 0, 0.001, 0.01, 0.1, and 0.5 U/ml rhArg, respectively. Y-axis of histogram represents number of cells analyzed, and x-axis represents function of fluorescence intensity resulted from binding of FITC-dUTP (fluorescein isothiocyanate tagged deoxyuridine triphosphate) to fragmented DNA.

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