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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: Opposite regulation by PI3K/Akt and MAPK/ERK pathways of tissue factor expression, cell-associated procoagulant activity and invasiveness in MDA-MB-231 cells

Figure 4

TF promoter activity, TF mRNA and Akt phosphorilation after combined treatments. Panel a: MDA-MB-231-TFluc cells were treated by 0.1 μM of erlotinib in the absence or the presence of 10 μM PD98059 for 24 hrs. The cell activity of luciferase was measured. The data was from three independent experiments. Panel b: qPCR results of TF mRNA in the cells after the treatment for 24 hrs by 50 nM of cetuximab or 0.1 μM of erlotinib in the absence or the presence of 10 μM PD98059. The data were from 3 independent experiments. * : p<0.05. Panel c: The western blots of TF protein in the cells treated for 24 hrs by 10 μM PD98059, 10 μM of A6730, 50 nM of cetuximab and 0.1 μM of erlotinib. The data were from 3 blots. * : p<0.05 in comparison with control. Panel d: The western blots of TF protein in the cells treated for 24 hrs by 10 μM LY294002, 0.1 μM erlotinib and 10 μM A6730 in the presence or the absence of 10 μM PD98059. The data were from 3 blots. * : p<0.05.

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