Figure 2
Expression levels of TF mRNA and TF protein in treated MDA-MB-231 cells. Panel a: The qPCR results of total TF mRNA levels in treated MDA-MB-231 cells. The cells were treated for 24 hr by the indicated agents at the indicated concentrations. qPCR was performed with primers Hs00175225_m1. The results were obtained from three independent experiments. Statistical significance (p<0.05) was found for all of the groups in comparison with the control group, except for the group of 5 μM PD98058. Panel b: The western blot of TF protein levels in PD98059-treated cells, showing a dose dependent increase in TF levels at 24 hrs. Panel c: The western blot of TF protein levels in the cells treated by LY294002 (10 μM) and wortmannin (0.1 μM) at 24 hrs. The data of the ratio were obtained with 3 repeated blots. * : p<0.05 in comparison with the controls.