Figure 5
From: BCR-ABL1-independent PI3Kinase activation causing imatinib-resistance
Effect of imatinib and rapamycin on phosphorylation of STAT5 and RPS6. Cell lines were treated for 24 h with imatinib (1 μM), rapamycin (10 nM) or a combination of both agents. Phosphorylation of STAT5 and RPS6 was determined by Western blot analysis. Note that imatinib induces dephosphorylation of STAT5 and of RPS6 in TKI-sensitive cell lines, dephosphorylation of STAT5 in TKI-resistant cell lines. Rapamycin inhibits phosphorylation of RPS6; ima = imatinib; rapa = rapamycin.