Figure 1

Detection of JAK2V617F by allele-specific PCR. Nested PCR was performed with crude genomic DNA samples as described in Methods. PCR products were analyzed on 3% agarose and visualized by ethidium bromide staining. The expected PCR products are 453 bp (for both JAK2V617F-positive and -negative alleles), 279 bp (for JAK2V617F-positive allele), and 229 bp (for JAK2V617F-negative allele). Lane 1 was done with water in place of genomic DNA samples to rule out possible cross-contaminations. Lane 11 did not give a clear PCR product and was excluded from further analysis. Samples 2 and 9 are JAK2V617F-positive, while all the rest are JAK2V617F-negative.