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Figure 1 | Journal of Hematology & Oncology

Figure 1

From: Effect of a structurally modified human granulocyte colony stimulating factor, G-CSFa, on leukopenia in mice and monkeys

Figure 1

Analysis of expression and purification of recombinant G-CSFa. (A) G-CSFa was expressed and purified as described in Materials and Methods. Purified G-CSFa (lane 3) and bacterial cell lysates before (lane 1) and after (lane 2) IPTG addition were analyzed by SDS-PAGE. Lane M stands for molecular markers. Each experiment was repeated for at least three times and representative data are shown. (B) N-terminal amino acid sequences of G-CSFa determined by Edman degradation method. The mutated amino acid residues are highlighted in red.

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