Figure 2

Detection of CREB-1 and Sp1 protein obtained from the chromatin immunoprecipitation (ChIP). A ChIP protocol was performed to pull down the CREB-1 and Sp1 proteins from the individual cell lysates using antibodies against CREB-1 and Sp1 respectively. Half of the agarose beads obtained from the ChIP study were analyzed by western blots to determine the amount of the transcription factors precipitated from individual cell lysates. The remainder of the beads was analyzed by real time PCR to determine the amount of the XPC gene promoter DNA co-precipitated with the individual transcription factors.