CD7 MSP | CD7 mRNA negative | CD7 mRNA low | CD7 mRNA high |
|---|
U | 0 | 0 | 2 |
U/M | 1 | 2 | 11 |
M | 8 | 1 | 0 |
- CD7 methylation was assessed by MSP. U: products with primers recognizing unmethylated CD7 only; U/M: U and M (methylated) products; M: M products only. CD7 mRNA expression was assessed by qRT-PCR and evaluated as described in Table 2. Note that 2/2 unmethylated (U only) cell lines are CD7-positive and that 8/9 methylated (M only) cell lines are CD7-negative. Methylation-specific (M) and unmethylation-specific (U) PCR were very sensitive, allowing the detection of one methylated cell in 1000 unmethylated cells and vice versa. Therefore, a meaningful correlation between CD7 methylation and expression was not apparent in U/M cells.
