CD7 in acute myeloid leukemia: correlation with loss of wild-type CEBPA, consequence of epigenetic regulation

Table 2 C/EBPα and CD7 expression in individual AML cell lines

Cell lines	*CEBPA* mRNA	C/EBPα protein	*CD7* mRNA	CD7 protein
AP-1060	+	+	-	-
CMK	(+)	-	+	+
CMY	-	-	+	+
F-36P	-	-	+	+
GDM-1	(+)	-	+	+
HL-60	+	+	-	-
HNT-34	+	+	+	+
HT-93A	+	+	-	-
IMS-M1	+	+	+	-
KG-1	(+)	-	+	+
M-07e	-	-	+	+
ME-1	+	+	+	-
MEGAL	(+)	-	+	+
MOLM-16	-	-	+	+
MONO-MAC-6	+	+	(+)	-
MUTZ-8	+	+	(+)	(+)
NB-4	+	+	-	-
OCI-AML2	+	+	-	-
OCI-AML5	+	+	-	-
OCI-M1	(+)	(+)	-	(+)
OCI-M2	(+)	-	-	(+)
SET-2	(+)	(+)	+	+
SIG-M5	+	+	-	-
SKNO-1	(+)	(+)	(+)	-
TF-1	-	-	+	+

C/EBPα and CD7 Western blot analysis: + positive, (+) weakly positive, - negative. GAPDH was used as protein loading control. CEBPA qRT-PCR: +: 2^-ΔΔct ≥ 5.0; (+) 2^-ΔΔct ≥ 0.2, < 5.0; -: 2^-ΔΔct < 0.2. The CEBPA-low cell line SET-2 was used as calibrator cell line. CD7 qRT-PCR: +: 2^-ΔΔct > 2.5; (+) 2^-ΔΔct ≥ 1; -: 2^-ΔΔct < 1. The CD7-low cell line MUTZ-8 was used as calibrator cell line. Note that cell lines HNT-34, IMS-M1 and ME-1 (bold) express C/EBPα and CD7 mRNA.

ISSN: 1756-8722