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Figure 4 | Journal of Hematology & Oncology

Figure 4

From: SMI of Bcl-2 TW-37 is active across a spectrum of B-cell tumors irrespective of their proliferative and differentiation status

Figure 4

Inventory of Bcl-2 family protein by Western-blot quantification of anti-, pro-apoptotic Bcl-2 family protein of 4 NHL cell lines (4.A) and 5 fresh patient derived samples (4.B). Cells were harvested and lysed for Western-blot analysis. Forty μg of total lysate was subjected to detect multi-domain anti-apoptotic proteins Bcl-2, Bcl-XL and Mcl-1 proteins in NHL cell lines and patient derived samples. 80 μg of total cell lysate was loaded to detect multi-domain pro-apoptotic and BH3-only Bax, Bak, Bok, Bad, Bim and Puma profiles in WSU cell lines and patient-derived fresh samples.

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