Figure 7
Memory and effector T cell analyses of DC-activated immune effector cells. (A) & (B) Memory and effector phenotype analysis of a CD4 dominant donor (A) and a CD8 dominant donor (B). The DC-activated cells from day 0, 12, and 17 after coculture were analyzed for CD3, CD4, CD8, CD27, CD28, CD45RA, and CCR7 using a seven-color panel of fluorochrome-labeled Ab with FACSAria. The cells were gated for CD3 and CD4 or CD8 as total cells for the percentage analysis. One representative flow graph of 5 performed experiments is presented.