Figure 6From: Serum proteomic profiling and haptoglobin polymorphisms in patients with GVHD after allogeneic hematopoietic cell transplantationHaptoglobin genotyping. Hp genotyping based on combination of the results of two separate DNA amplification reactions involving primers A and B in the first reaction (lanes 2, 4, 6) and primers C and D in the second reaction (lanes 3, 5, 7). The reactions in lanes 2 and 3 contained DNA from the individual with genotype Hp 1-1; the reactions in lanes 4 and 5 contained DNA from the individual with genotype Hp 2-1; the reactions in lanes 6 and 7 contained DNA from the individual with genotype Hp 2-2. Lane 1, DNA size marker 100 – 1500 bp (Genscrip); lane 2, allele Hp 1; lane 3, no amplification product was obtained with the Hp 2-specific primer pair C/D because this sample was homozygous for allele Hp 1; lane 4, allele Hp 1; lane 5, allele Hp 2; lane 6, allele Hp 2; lane 7, allele Hp 2; lane 8, DNA size marker 1 kb plus (Gibco).Back to article page