Figure 1

Determination of the competitive binding of the Oct-1 decoy oligonucleotide in vitro. The radioactively labeled Oct-1 consensus DNA probe was incubated with HeLa nuclear extracts at room temperature for one hour and then analyzed by polyacrylamide gel electrophoresis using a 6% gel. Some reactions also contained the unlabeled Oct-1 decoy oligonucleotide or the scrambled oligonucleotide for competitive binding. The Oct-1 binding-caused gel mobility shift was confirmed by a western blot hybridization assay using an Oct-1 antibody (data not shown).