Figure 1

PHI suppresses growth and causes cell cycle arrest of RPMI8226 myeloma cells. (A). PHI suppresses growth of RPMI8226 myeloma cells. The myeloma cells were cultured with or without phenylhexyl isothiocyanate (PHI) at varying concentrations for 24, 48 or 72 hours. The cell number was recorded at each time point. Diamond (♦), control; Square (■), 0.1 μM; Triangle (), 0.5 μM; Dot (●); 5.0 μM. (B). PHI induces G1 growth arrest. The myeloma cells were cultured with 0.5 μM of PHI for 48 or 96 hours. The cellular DNA content was determined by flow cytometry. Values are means +/- SD from 3 independent experiments. Open bar, G1 phase; Black bar, G2M phase; Grid bar, S phase.