Figure 2
Effects of LA on cell cycle phase distribution and the expression of various cell cycle regulatory proteins in HL-60 cells. (A) Cells were treated with 0, 2.5 and 5 mM LA for 24 and 48 h and analyzed by flow cytometry. Cells with hypodiploid DNA content (sub-G1) represent apoptotic cell fractions. (B) Western blot analysis of total Rb, pRB (ser780), pRB (ser 807/811) and E2F expression in cell lysate treated with LA for 48 h. (C) The level of immunoreactive cyclins B1, E, cdk1 and cdk2 in LA-treated HL-60. The intensity of the specific immunoreactive bands were quantified by densitometry and expressed as a fold difference against actin.