Figure 1

CD34 selection efficiency by aldefluor assay and flow-cytometry assay. After CD34 selection, cells were stained with CD34-PE and Aldefluor. CD34+ stem cells were selected according to forward scatter (FSC) and side scatter (SSC) properties using the gated region R1 to remove cell debris, residual platelet and red cell contamination. Figures were representative of 6 separate experiments. A: Phenotype of day 0 CD34⁺ selected cells that initiate the culture. The percentages of cells positive for the CD34-PE and Bodipy-aminoacetate (BAAA) were obtained from gates R1. B: Isotype expression pattern for IgG-PE and BAAA. C: Expression profile of stem cells co-staining with CD34-PE and BAAA. As shown in the Fig, based on R1 gating, 81.05% cells were both CD34 positive and BAAA brightly stained, 12.65% cells were CD34 positive but BAAA negative. 86.50% of the CD34+ cells were brightly stained with BAAA.